点评详情
发布于:2018-2-14 02:08:25  访问:16 次 回复:0 篇
版主管理 | 推荐 | 删除 | 删除并扣分
br Methodology The study was approved by
SPR analysis of small molecule binding to immobilized NGF in the presence of 1% BSA was found to be statistically correlated to values determined through 125I-NGF binding (Fig. 3). This relationship is not statistically significant in the absence of BSA. Thus, it is possible that BSA acts as a carrier protein for the small Lomustine for increased affinity for NGF. It is hypothesized that BSA binds small molecule compounds, enabling a small axial rotation of the compound‘s structure, placing the small molecule in a conformation ideal for high affinity binding to available NGF.


Conclusions
SPR cell-free techniques provide an advantage in drug development by eliminating other potentially influencing proteins traditionally found in cell-based binding assays. By introducing proteins independently, SPR allows for a more clear understanding of the binding events which occur in vitro affecting drug activity. By identifying carrier proteins which participate in small molecule binding to NGF the development of future pain therapeutics will evolve to account for such protein interactions in vivo. BSA has shown to alter small molecule binding to immobilized NGF in a cell-free system. This shift in binding affinity for NGF in the presence of BSA eliminates the previously observed variance in binding measurements when compared to cell-based assays. These results suggest that BSA plays a critical role in small molecule binding efficacy to NGF in vitro. In addition, these results will allow tailoring future development strategies to account for the influence of carrier proteins to identify therapeutic lead agents.


Funding


Index terms


Introduction
Modern photonic technology is developing in an incredible pace in sensor field after the invention of novel photonic crystal fiber. Photonic crystal fiber becomes a potential nominee in the sensor field due to its most intriguing structure which support band gap and modified total internal reflection unique propagation phenomena that is not possible in conventional fiber system. This micro structure holey fiber has some prime factor which supports unique sensing properties such as (i) Photonic crystal fiber made from single silica material. (ii) Various array design of air hole. (iii) Infiltration of sensing liquid inside air hole adds extra degree of freedom for sensing application [1]. Currently fabrication of PCF sensor is in mature level and various fabrication techniques such as capillary stack and drawing, sol-gel casting, drilling, slurry casting and extrusion method support sub-micron scale fabrication. Commercialization of PCF sensor accepted by various application such as food safety, liquid and gas detection, medical detection, biosensing, bioimaging and drug detection [2]. PCF sensor has silent feature such high sensitivity, high performance, low cost and flexible design because PCF sensing involve light matter interaction by filling sensing liquid in core region.
In 1996, Knight et al. [3] fabricate first hexagonal PCF design and better sensing has been achieved by number of geometrical design like octagonal [4], decagonal [5], elliptical [6], honey comb cladding [7], hybrid core cladding [8] and hexagonal [9] etc. Further, porous PCF fiber [10] also draws the attention due high birefringence, minimum confinement loss and flexibility in fabrication design. Various form of porous PCF design have been reported such as porous horizontal and vertical slotted core [11,15], porous circular and elliptical air hole [12–13], porous diamond core [14], porous hexagonal core [16–17]. Recent, spiral topology [18] also has been gained attenuation due excellent modal confinement property. Spiral cladding [19–20] pattern consist spiral arm and each arm has definite number of air hole. As a result, all these reported PCF sensor analysed either analytically or numerically. Finite element method (FEM) is numerically technique to solve the Maxwell equation with proper boundary condition. Moreover, relative sensitivity and confinement loss are analytically approach (mathematically approach) to measure the sensor performance. This proposed sensor simulatively detects various biochemical analyte having refractive index range 1.50–1.56 [24–25]. Photonic sensing technology opens new measurement possibility with accuracy because chemical and biochemical analyte have different permittivity more than water and air. So velocity of propagation decrease due to interaction with biochemical molecules resulting light signal measured quantify with presence of analyte and further used for chemical and biomedical applications. The motivation behind for detection of chemical and biochemical analyte is because many lives can be saved if disease tissues and harmful chemical detected at an early stage. Conventional detection techniques are tedious, time consuming and very painful to patient. On other hand Photonic sensor (lap on chip sensor) are more reliable, accurate, cost effective, and smaller in footprint and more compatible to human body. The ultimate aim of this research to design a lab on chip sensor based on photonic crystal fiber for detection of biochemical. To best of our knowledge, various porous core designs has been reported before but no one discuss this porous concept with multiple circular sensing rings with spiral cladding pattern.
共0篇回复 每页10篇 页次:1/1
共0篇回复 每页10篇 页次:1/1
我要回复
回复内容
验 证 码
看不清?更换一张
匿名发表 
当前位置
脚注信息
版权所有 Copyright(C)2009-2017 北京众康中医养生堂专业调理