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St 8 hours for 3 days, the minimum required to be included in
St 8 hours for 3 days, the minimum required to be included in the study.17 19 Statistical analyses Full details of the analysis plan have been published previously.19 Briefly, main analyses assessing the effect of the Title Loaded From File Title Loaded From File intervention on the primary and secondary 12 months postintervention were conducted as intention-to-treat, with missing data at baseline being replaced with a value of 999 and a variable to indicate missing data at baseline (0=not missing, 1=missing) being included in regression models, as recommended by White et al.24?6 For primary outcomes, the level of statistical significance used was p<0.05 and for secondary outcomes, the level of statistical significance used was p<0.01, after correcting for multiple testing.19 A series of sensitivity analyses were conducted to test assumptions regarding the nature of missing data at baseline and at each of the follow-up assessments (see detailed analysis plan19 for discussion of these assumptions and the sensitivity analyses). Multilevel regression models were used to account for clustering (non-independence) of children within schools.19 All analyses included adjustment for the following baseline variables: age, sex, baseline measure of the outcome being analysed, involvement in other healthy behaviour promoting activities and school level deprivation. A secondary per-protocol analysis was undertaken, in which classes in the intervention arm were only included in analyses if teachers had taught at least 70 (11 of 16) of the AFLY5 lessons. There was one school for which we were unable to confirm how many lessons had been taught. For that school, we first did analyses assuming that they had been taught at least 11 lessons and then repeated them assuming that they had been taught fewer than 11; the results were identical whichever of these alternatives were used. We additionally assessed whether the effect of the intervention on accelerometer-assessed outcomes differed by week orPrimary outcomes Accelerometer-assessed mean time per day spent doing moderate/vigorous physical activity MVPA (min/day) Accelerometer-assessed mean time per day spent in sedentary activity (min/day) Self-reported (validated questionnaire) servings of fruit and vegetables consumed per day (servings per day; treated in all analyses as a continuous variable) Secondary outcomes Self-reported (validated questionnaire) mean time spent screen viewing on a typical weekday (min) Self-reported (validated questionnaire) mean time spent screen viewing on a typical weekend day (min) Self-reported (validated questionnaire) servings of snacks consumed per day (servings per day; treated in all analyses as a continuous variable) Self-reported (validated questionnaire) servings of high-fat foods consumed per day (servings per day; treated in all analyses as a continuous variable) Self-reported (validated questionnaire) servings of high-energy drinks consumed per day (servings per day; treated in all analyses as a continuous variable) Body mass index determined from weight and height measured in classrooms by two study fieldworkers (kg/m2; treated in all analyses as an SD z-score) Waist circumference measured in classrooms by two study fieldworkers (mm; treated in all analyses as an SD z-score) General overweight/obesity, determined by the International Obesity Task Force thresholds of body mass index for children (taking account of their age and sex) (binary outcome) Central overweight/obesity determined by thresholds of UK a.St 8 hours for 3 days, the minimum required to be included in the study.17 19 Statistical analyses Full details of the analysis plan have been published previously.19 Briefly, main analyses assessing the effect of the intervention on the primary and secondary 12 months postintervention were conducted as intention-to-treat, with missing data at baseline being replaced with a value of 999 and a variable to indicate missing data at baseline (0=not missing, 1=missing) being included in regression models, as recommended by White et al.24?6 For primary outcomes, the level of statistical significance used was p<0.05 and for secondary outcomes, the level of statistical significance used was p<0.01, after correcting for multiple testing.19 A series of sensitivity analyses were conducted to test assumptions regarding the nature of missing data at baseline and at each of the follow-up assessments (see detailed analysis plan19 for discussion of these assumptions and the sensitivity analyses).
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